Fig. 2

Expression of Cyr61, autophagy factors, and podocyte markers in podocytes regulated by HOXA11-OS. A HOXA11-OS mRNA expression was detected by qRT-PCR after HOXA11-OS overexpression and knockdown lentiviruses were successfully transfected into podocytes. ^*P < 0.001 vs. ex-NC; ^*P < 0.001 vs. sh-NC. B After HOXA11-OS overexpression and knockdown lentiviruses were successfully transfected into podocytes, the expression of Cyr61, Beclin-1, LC3B, nephrin, and podocin was detected by qRT-PCR and western blotting. ^*P < 0.01 vs. ex-NC; ^#P < 0.01 vs. sh-NC. C After HOXA11-OS overexpression and knockdown lentiviruses were successfully transfected into podocytes, the localization and expression of Cyr61, LC3B, and nephrin proteins in each group were detected by IF. Relative average optical density; ^*P < 0.01 vs. ex-NC; ^#P < 0.01 vs. sh-NC. Scale bars represent 15 µm