Fig. 3

miR-124-3p directly binds to the 3’ untranslated regions of HOXA11-OS and Cyr61 in podocytes. A The subcellular localization of HOXA11-OS was detected by FISH assay. The expression of 18S, U6, and HOXA11-OS were similar, and there was no statistical difference in optical density. B The expression of miR-124-3p in the kidney tissue of lupus mice and podocyte injury model was detected by qRT-PCR. ^*P < 0.01 vs. control group. C The expression of HOXA11-OS in miR-124-3p mimic or inhibition cell lines and the expression of miR-124-3p in HOXA11-OS overexpression or knockdown cell lines were detected by qRT-PCR. ^*P < 0.001 vs. miR-NC/ex-NC/sh-NC. D The binding sites of miR-124-3p on HOXA11-OS and Cyr61 in HOXA11-OS-wild-type (WT) and Cyr61-WT, and the mutation sites in HOXA11-OS-mutant (MUT) and Cyr61-MUT are indicated in red. Bi-luciferase reporter genes were detected 48 h after transfection of HOXA11-OS-WT, Cyr61-WT, HOXA11-OS-MUT, or Cyr61-MUT and miR-124-3p mimic or miR-NC. ^**P < 0.01 vs. HOXA11-OS-WT + miR-NC/Cyr61-WT + miR-NC