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Fig. 2 | Molecular Medicine

Fig. 2

From: OR2AT4 and OR1A2 counterregulate molecular pathophysiological processes of steroid-resistant inflammatory lung diseases in human alveolar macrophages

Fig. 2

OR2AT4 and OR1A2 are expressed in alveolar macrophages. Transcripts of OR2AT4 (A), OR1A2 (B), and OR1A1 (C) were analyzed by RT-PCR. “+” or “−“ indicate the presence and absence (negative control) of the reverse transcriptase in the enzyme mix. The estimated sizes of OR2AT4, OR1A2, or OR1A1 were 400, 212, or 670 bp (n = 3). OR2AT4 (D) and OR1A2 (E) proteins were detected by Western blot (n = 2–3). The estimated size of both OR2AT4 and OR1A2 was 34 kDa (arrows). F, G Intracellular localization of OR2AT4 and OR1A2 (each in red) by immunocytochemistry (n = 3). The plasma membrane was stained by an antibody against E-cadherin (green). Hoechst 33,342 marked the nucleus (blue). The merge shows the overlay of all markers. Bars indicate a range of 25 µm and squares mark the zoomed area. (n = 3)

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Molecular Medicine

ISSN: 1528-3658