Fig. 3

Sandalore mediates an increase of intracellular calcium via a cAMP-dependent pathway. AM were stimulated three times for 1 min each with 300 µM Sandalore (A, dark grey). Intermediary washing steps removed the odorant or inhibitor. B Repetitive application of 300 µM Sandalore with co-application of 300 µM Oxyphenylon (second application, light grey). EGTA 10 mM (C) and MDL-12,330A 10 μM (D) were pre-incubated for three (EGTA) or five minutes (MDL 12,330A) before adding Sandalore in the second application (light grey). Data are shown as mean ± SEM (n = 9 of 3 donors). Data were analyzed with the Student’s paired t-test referring to the third Ca²⁺ amplitude induced by Sandalore. *p ≤ 0.05 and **p ≤ 0.01