Fig. 3From: Icaritin inhibits neuroinflammation in a rat cerebral ischemia model by regulating microglial polarization through the GPER–ERK–NF-κB signaling pathwayThe effect of ICT on activation and polarization of microglia in tMCAO rats. A and B Representative immunofluorescent images of microglia polarization in the ischemic penumbra. The immunofluorescence staining of microglia with anti-CD68 (magenta), CD206 (magenta), and Iba1 (green) antibody. The nucleus was stained by DAPI; scale bar = 10 μm. C–E Immunofluorescence quantitation (n = 3). F–I The protein expressions of Iba1, CD40 and CD206 (n = 4). J–L The mRNA expression of CD11b, CD68, and CD206 (n = 6). The results are expressed as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001Back to article page