Fig. 3
From: Exenatide regulates Th17/Treg balance via PI3K/Akt/FoxO1 pathway in db/db mice
Detection of Th17 cells in pancreas from control (Con) and exenatide (Exe) groups at week 0 and week 8. A mRNA levels of IL-17 by real-time PCR. mRNA quantities were calculated as a ratio to the level of GAPDH mRNA in each sample. Data are shown as the relative expression ratio to Control 0 Week. Results are expressed as mean ± SEM, n = 8 in each group. B Representative image and gray value analysis of IL-17 by western blot. ACTIN was used as a loading control. Data are shown as the relative expression ratio to Control 0 Week and expressed as mean ± SEM, n = 8 in each group. C Representative immunofluorescence of pancreatic sections using anti-IL-17 staining (red), anti-insulin staining (green) and DAPI staining (blue), scale bar = 40 μm, n = 8 in each group. *P < 0.05 vs control 0 week; ns P > 0.05