Fig. 5

CD44 promotes plasma exosome proangiogenic function by enhancing FGFR2 signaling transduction. A, B Western blot analysis of the activation of the FGFR2 pathway after 48 h coculture of WT MVECs with WTSham plasma exosomes (WT + WTSham-Exo), KO MVECs with KOSham plasma exosomes (KO + KOSham-Exo), WT MVECs with WTMI plasma exosome (WT + WTMI-Exo), KO MVECs with KOMI plasma exosomes (KO + KOMI-Exo), WT MVECs with KOMI plasma exosomes (WT + KOMI-Exo), and KO MVECs with WTMI plasma exosomes (KO + WTMI-Exo), respectively. n = 3. C The tube formation assay was used to test the angiogenic function of plasma exosomes (WTSham-Exo, KOMISham-Exo, WTMI-Exo, and KOMI-Exo) in WT and KO MVECs. Scar bar: 50 μm. D The statistical data of the number of nodes (Nb Nodes) and the number of junctions (Nb Junctions). n = 3. E CD44 WT and KO MVECs were co-cultured with WTSham-Exo, KOSham-Exo, WTMI-Exo, and KOMI-Exo for 48 h, and the ability of cell migration was detected through the Transwell migration assay. Scale bar: 100 μm. F The numbers of cells (Nb Cells) that migrated through the inserts were counted and compared between groups statistically. n = 3. *P < 0.05, **P < 0.01, ***P < 0.001