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Fig. 2 | Molecular Medicine

Fig. 2

From: OLMALINC/OCT4/BMP2 axis enhances osteogenic-like phenotype of renal interstitial fibroblasts to participate in Randall’s plaque formation

Fig. 2

OCT4 enhanced osteogenic-like differentiation of human renal interstitial fibroblasts (hRIFs) in vitro. A Representative immunofluorescence co-staining images of Vimentin (Green) and OCT4 (Red) in Randall’s plaques (RP; n = 6) and normal renal papillae (NRP; n = 6). B Immunoblotting (IB) analysis of OCT4 in hRIFs 0, 3, 7, and 14 days after osteogenic medium (OM) induction (n = 3). C HRIFs were transfected with recombinant lentivirus to overexpress or silence OCT4, and qRT-PCR determined the efficiency (n = 3). Len-sh3-OCT4 was selected for silencing OCT4 in the following experiments because of the highest efficiency. D Alkaline phosphatase (ALP) activity in cell lysis of transfected hRIFs 7 days after osteogenic induction (n = 3). E, F IB analysis of OCT4, RUNX2, and OCN in OCT4 silenced hRIFs 7 days after osteogenic induction (n = 3). G, H IB analysis of OCT4, RUNX2, and OCN in OCT4 overexpressed hRIFs 7 days after osteogenic induction (n = 3). I Alizarin Red Staining (ARS) for calcium nodes in transfected hRIFs 14 days after osteogenic induction (n = 3)

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Molecular Medicine

ISSN: 1528-3658