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Fig. 3 | Molecular Medicine

Fig. 3

From: OLMALINC/OCT4/BMP2 axis enhances osteogenic-like phenotype of renal interstitial fibroblasts to participate in Randall’s plaque formation

Fig. 3

OCT4 enhanced osteogenic-like differentiation of human renal interstitial fibroblasts (hRIFs) in vivo. A The porous structure of bone mineral substitute granules visualized by scanning electron microscopy (X20000). B A representative fluorescence image of a nude female mouse 8 weeks after subcutaneous implantations carrying transfected hRIFs expressing luciferase (n = 3). C HE and Masson staining of serial sections from the blank group (granules without cells), the normal control (NC) group (granules with hRIFs), the Len-ctrl group (granules with hRIFs transfected with Len-ctrl), and the Len-OCT4 group (granules with hRIFs transfected with Len-OCT4), Len-sh-ctrl group (granules with hRIFs transfected with Len-sh-ctrl) and the Len-sh-OCT4 group (granules with hRIFs transfected with Len-sh-OCT4). IHC (OCT4; RUNX2; OCN) staining of serial sections from the NC group, the Len-ctrl group and the Len-OCT4 group, Len-sh-ctrl group, and the Len-sh-OCT4 group. Each of the 6 groups was implanted in 1 subcutaneous site of 6 mice and collected 8 weeks after implantation

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Molecular Medicine

ISSN: 1528-3658