Fig. 2
From: Zebrafish: an efficient vertebrate model for understanding role of gut microbiota
Overview of generation procedures for germ-free zebrafish. On day -1, pairs of adult zebrafish are placed in breeding cages overnight, with males and females separated using a divider. The next morning (Day 0), embryos are collected, rinsed, and transferred to a petri dish containing a filter-sterilized embryo medium. Three general methods are used to obtain gnotobiotic zebrafish embryos, including natural breeding, laparotomy, and squeezing. After the larvae are transferred into sterile culture flasks or gnotobiotic isolator, they are maintained in an incubator to develop at 28.5 °C. On 4 dpf, GF zebrafish colonized by microbiota