Fig. 1

LOTUS inhibits Aβ-PirB binding. a Representative images of the binding assay in Cos7 cells overexpressing LOTUS or PirB. The cells were treated with biotin-conjugated-human Aβ and immunostained using LOTUS or PirB antibody without Aβ. Scale bars, 100 µm. b Quantification of cell surface expression level of PirB in Cos7 cells overexpressing PirB and/or LOTUS. Each expression level of PirB was normalized to the expression level in Cos7 cells overexpressing PirB alone, and the data are presented as mean ± standard error of the mean (SEM) from four independent cell culture preparations (n = 4, one way analysis of variance ANOVA post hoc Tukey test n.s., not significant). c Quantification of Aβ binding in Cos7 cells overexpressing LOTUS and/or PirB. Aβ binding levels in each group. Binding levels were normalized to the binding level of Aβ (100 nM) in Cos7 cells overexpressing PirB alone. The data are presented as mean ± SEM (n = 7, One-way ANOVA post hoc Tukey test *p < 0.05, **p < 0.01)