Fig. 5

Human LOTUS inhibits Aβ binding to LilrB2. a Representative images of the binding assay in Cos7 cells overexpressing human LOTUS or LilrB2. The cells were treated with biotin-conjugated-human Aβ and immunostained using LOTUS or PirB antibody without Aβ. Scale bars, 100 µm. b Quantification of cell surface expression level of LilrB2 in Cos7 cells overexpressing human LOTUS and/or LilrB2. Each expression level of LilrB2 was normalized to the expression level in Cos7 cells overexpressing LilrB2 alone, and the data are presented as mean ± SEM from four independent cell culture preparations (n = 4, one-way ANOVA post hoc Tukey test n.s., not significant). c Quantification of Aβ binding in Cos7 cells overexpressing LOTUS or LilrB2. Aβ binding levels in each group were normalized to the binding level of Aβ (100 nM) in Cos7 cells overexpressing PirB alone, and the data are presented as mean ± SEM (n = 5, One-way ANOVA post hoc Tukey test *p < 0.05, **p < 0.01)