Fig. 5
Nine subpopulations of monocytes and 7 subpopulations of macrophages were identified with different markers and functions. A t-SNE plot showed 9 subpopulations of monocytes upon re-clustering. B the proportion for each subpopulation of monocytes between control and ATAD group. The dashed line represented the highest proportion of Mono 8 in control group. C heatmap of marker genes for each subpopulation of monocytes. D t-SNE plots showed the expressions of representative marker genes for each subpopulation of monocytes. E Qusage analysis of hallmark gene-sets enrichment for subpopulations of monocytes. F the expressions of cytokines for subpopulations of monocytes. G t-SNE plot exhibited 7 subpopulations of macrophages. H The proportion for each subpopulation of macrophages between control and ATAD group. The dashed line was used to discriminate the dominant subpopulations in ATAD and control group. I Heatmap of marker genes for each subpopulation of macrophages. J t-SNE plots revealed the expressions of representative marker genes for each subpopulation of macrophages. K Qusage analysis of hallmark gene-sets enrichment for each subpopulation of macrophages. L the expressions of cytokines for subpopulations of macrophages. M DEGs and GO analysis for up-regulated and down-regulated genes in subpopulations of monocytes (ATAD/Control). N DEGs and GO analysis for up-regulated and down-regulated genes in subpopulations of macrophages (ATAD/Control)