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Fig. 6 | Molecular Medicine

Fig. 6

From: Single-cell transcriptomic analysis reveals differential cell subpopulations and distinct phenotype transition in normal and dissected ascending aorta

Fig. 6

The interaction among VSMCs, FBs and neutrophils in control and ATAD group as well as cell differentiation trajectory of FBs. A t-SNE plots exhibited the expressions of CXCL12, CXCR4 and ACKR3 in all cell types. B t-SNE plots exhibited the expressions of CXCR4 and ACKR3 in neutrophils and FBs. C IHC revealed higher expression of CXCR4 in CD11b+ cells for media of ATAD group and ACKR3 in DCN+ cells for adventitia of ATAD group. D the predicted downregulation targets upon the combination of CXCL12 with CXCR4/ACKR3. E CytoTRACE predicted the cell differentiation potential of FBs. F genes correlated with more differentiated and less differentiated cells predicted by CytoTRACE. G pseudo-time analysis showed the cell differentiation trajectory of FBs upon selecting FBs 7 as the initiate. H the heatmap of gene expression alteration relating to ECM organization and cell proliferation. I the alteration curves of representative genes. The full line represented cell fate 1, the dashed line represented cell fate 2. J IHC showed higher expressions of ENO1 in DCN+ FBs from ATAD adventitia and higher proportion of CXCL12+/STEAP4+ cells in adventitia of ATAD group. K IF verified the higher expressions of ENO1 and STEAP4 in DCN+ FBs from ATAD adventitia. L heatmap showed similar expressions of marker genes between FBs 8 (synthetic VSMCs-like FBs) and VSMCs 1 (synthetic VSMCs) and distinct markers of FBs 7 and VSMCs 2

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Molecular Medicine

ISSN: 1528-3658