Fig. 1

Ischemia–reperfusion injury induced lncRNA uc007nnj.1 expression of in vivo and in vitro. Cultured primary mouse RGCs were treated with or without Ca²⁺ (2µM) and antimycin (10 µM) in HBSS (ATP and glucose depletion) at indicated time periods (ischemia/reperfusion for 0/0 h, 2/0 h, 2/2 h, and 2/4 h, respectively). Reverse transcription-quantitative real-time PCR (RT-qPCR) analysis was used to detect the expression of lncRNA uc007nnj.1. B The intraocular pressure was elevated to 120 mmHg for 1 h to induce transient retinal ischemia and then exposed to reperfusion for 6, 12, 24, and 48 h in C57BL/6J mice. The expression levels of lncRNA uc007nnj.1 in the retinas were detected by RT-qPCR. C Representative FISH images of the location of lncRNA uc007nnj.1 (red) in RGCs. U6 and 18 s were used as nuclear and cytoplasmic markers, respectively. Scale bar: 10 µm. D Relative intensity analysis of FISH images. Data are expressed as mean ± SD of six independent experiments; ^#p < 0.05 versus 0/0 h or sham group