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Fig. 2 | Molecular Medicine

Fig. 2

From: Long noncoding RNA uc007nnj.1 mediates neuronal death induced by retinal ischemia/reperfusion in mice via the miR-155-5p/Tle4 axis

Fig. 2

lncRNA uc007nnj.1 deletion alleviated ischemia-induced neuronal apoptosis. RGCs were transfected with 50 nM scramble siRNA or lncRNA uc007nnj.1 siRNA for 24 h and then treated with or without I/R injury. A RT-qPCR analysis of the expression of lncRNA uc007nnj.1. B, C Measurement of total apoptosis by flow cytometry in RGCs. The Q1 area represents necrotic cells, the Q2 area represents late apoptotic cells, the Q3 area represents early apoptotic cells, and the Q4 area represents normal cells. D Representative image showing a Western blot of caspase-3 and cleaved caspases-3. E, F Quantitative analysis of immunoreactive bands of caspase-3 (E) and cleaved caspase-3 (F). Data are expressed as mean ± SD (n = 6). #p < 0.05, scramble with I/R group or lncRNA uc007nnj.1 siRNA group versus scramble group; *p < 0.05, lncRNA uc007nnj.1 siRNA with I/R group versus scramble with I/R group

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Molecular Medicine

ISSN: 1528-3658