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Fig. 3 | Molecular Medicine

Fig. 3

From: Long noncoding RNA uc007nnj.1 mediates neuronal death induced by retinal ischemia/reperfusion in mice via the miR-155-5p/Tle4 axis

Fig. 3

Overexpression of lncRNA uc007nnj.1 aggravated ischemia-induced neuronal apoptosis. RGCs were transfected with 1 g/mL lncRNA uc007nnj.1 plasmid or scramble and then treated with or without I/R. A RT-qPCR analysis of lncRNA uc007nnj.1 expression. B, C Representative data from the flow cytometric analysis of total cell apoptosis. The Q1 region represents necrotic cells, the Q2 region represents late apoptotic cells, the Q3 region represents early apoptotic cells, and the Q4 region represents normal cells. D Representative image showing a Western blot of caspase-3 and cleaved caspases-3. E, F Quantitative analysis of immunoreactive bands of caspase-3 (E) and cleaved caspase-3 (F). Data are expressed as mean ± SD (n = 6). #p < 0.05, scramble with I/R group or lncRNA uc007nnj.1 plasmid group versus scramble group; *p < 0.05, lncRNA uc007nnj.1 plasmid with I/R group versus scramble with I/R group

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Molecular Medicine

ISSN: 1528-3658