Fig. 4

lncRNA uc007nnj.1 mediates ischemia-induced RGCs apoptosis by regulating miR-155-5p as a ceRNA. A An analysis of the sequence alignment revealed lncRNA uc007nnj.1 contained potential complementary binding sequences for miR-155-5p. B Luciferase activities were measured in RGCs after being co-transfected with lncRNA uc007nnj.1 WT or lncRNA uc007nnj.1 MUT with miR-155-5p mimic or scramble. C Intracellular colocalization of lncRNA uc007nnj.1 and miR-155-5p in RGCs treated with or without I/R damage. Scale bar: 10 µm. RGCs were transfected with 50 nmol/L lncRNA uc007nnj.1 siRNA or 1 g/mL lncRNA uc007nnj.1 plasmid or scramble and then treated with or without I/R damage. D, E RT-qPCR analyzed the expression of miR-155-5p. Data are expressed as mean ± SD (n = 6). ^#p < 0.05, scramble with I/R group or lncRNA uc007nnj.1 siRNA group or lncRNA uc007nnj.1 plasmid group versus scramble group, lncRNA uc007nnj.1 WT/miR-155-5p mimic group versus lncRNA uc007nnj.1 WT group; *p < 0.05, lncRNA uc007nnj.1 siRNA with I/R group or lncRNA uc007nnj.1 plasmid with I/R group versus scramble with I/R group, lncRNA uc007nnj.1 MUT/miR-155-5p mimic group versus lncRNA uc007nnj.1 WT/miR-155-5p mimic group