Fig. 5

Ischemia-induced apoptosis was attenuated by miR-155-5p mimic. RGCs were transfected with 50 nM miR-155-5p mimic or miR-155-5p inhibitor or scramble and then with or without I/R treatment. A RT-qPCR analysis of miR-155-5p expression. B, C Representative data from the flow cytometric analysis of total cell apoptosis. The Q1 region represents necrotic cells, the Q2 region represents late apoptotic cells, the Q3 region represents early apoptotic cells, and the Q4 region represents normal cells. D Representative image showing a Western blot of caspase-3 and cleaved caspases-3. E, F Quantitative analysis of immunoreactive bands of caspase-3 (E) and cleaved caspase-3 (F). G RT-qPCR analysis of miR-155-5p expression. H, I Representative flow cytometric data and statistical data analysis of total cell apoptosis. J–L The apoptosis-related protein caspase-3 and cleaved caspase-3 were analyzed by Western blot. Data are expressed as mean ± SD (n = 6). ^#p < 0.05, scramble with I/R group versus scramble group; *p < 0.05, miR-155-5p mimic with I/R group or miR-155-5p inhibitor with I/R group versus scramble with I/R group