Fig. 6

Tle4 is a putative target of miR-155-5p. RGCs were transfected with 50 nM miR-155-5p mimic, Tle4 siRNA, or scramble before I/R of 2/2 h. A The putative target site for miR-155-5p within the 3′-UTR of mouse Tle4. B Detection of luciferase activity after co-transfection with 3′-UTR luciferase reporter vector for mouse Tle4-WT, Tle4-MUT, and miR-155-5p mimic. C, D RT-qPCR and Western blot analysis of Tle4 and β-actin. E Densitometric analysis of immunoblot bands. Data are expressed as mean ± SD (n = 6). ^#p < 0.05, scramble with I/R group or miR-155-5p mimic group versus scramble group, Tle4 WT/miR-155-5p mimic group versus Tle4 WT group; *p < 0.05, the miR-155-5p mimic group with I/R group versus scramble with I/R group, Tle4 MUT/miR-155-5p mimic group versus Tle4 WT/miR-155-5p mimic group