Fig. 7

Inhibition of Tle4 ameliorated I/R-induced apoptosis of RGCs. RGCs were transfected with 50 nmol/L Tle4 siRNA or scramble and then treated with or without I/R damage. A, B RGCs were treated with or without I/R at indicated time points (ischemia/reperfusion for 0/0, 2/0, 2/2, 2/4 h, respectively). The expression levels of Tle4 were detected by Western blot. C, D Representative data from the flow cytometric analysis of total cell apoptosis. The Q1 region represents necrotic cells, the Q2 region represents late apoptotic cells, the Q3 region represents early apoptotic cells, and the Q4 region represents normal cells. E, F Representative image and quantitative analysis of Western blot of cleaved caspase-3, caspase-3, and Tle4. Data are expressed as mean ± SD (n = 6). ^#p < 0.05, I/R for 0/0, 2/0, 2/2, 2/4 h versus 0/0 h, scramble with I/R group versus scramble group;^*p < 0.05, Tle4 siRNA group with I/R group versus scramble with I/R group