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Fig. 8 | Molecular Medicine

Fig. 8

From: Long noncoding RNA uc007nnj.1 mediates neuronal death induced by retinal ischemia/reperfusion in mice via the miR-155-5p/Tle4 axis

Fig. 8

lncRNA uc007nnj.1 knockdown attenuated ischemia-induced RGCs apoptosis via miR-155-5p. RGCs were co-transfected with 50 nM lncRNA uc007nnj.1 siRNA and anti-miRNA-155-5p or scramble and then treated with or without I/R. A, B RT-qPCR analysis of the expression levels of lncRNA uc007nnj.1 and miR-155-5p. C, D FCM analysis of total RGCs apoptosis. The Q1 region represents necrotic cells, the Q2 region represents late apoptotic cells, the Q3 region represents early apoptotic cells, and the Q4 region represents normal cells. E, F Western blot analysis for detection of activated caspase-3, caspase-3, and Tle4. Data are expressed as mean ± SD (n = 6). #p < 0.05, scramble with I/R group versus scramble group; *p < 0.05, lncRNA uc007nnj.1 siRNA with I/R group versus scramble with I/R group; ^p < 0.05, lncRNA uc007nnj.1 siRNA/Anti-miR-155-5p with I/R group versus lncRNA uc007nnj.1 siRNA with I/R group

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Molecular Medicine

ISSN: 1528-3658