Fig. 9
Suppression of lncRNA uc007nnj.1 ameliorated I/R-induced mouse retinal damage via miR-155-5p/Tle4 axis regulation. lncRNA uc007nnj.1 siRNA or scramble (1 μg/μL) was given to C57BL/6J mice vitreous 24 h before retinal I/R injury. A, B Representative immunolabeled images and quantitative analysis for anti-Tuj1 as RGCs marker in a magnified area of flat-mount retina 7 days after I/R treatment. Scale bar: 100 μm. C, D RT-qPCR analysis shows lncRNA uc007nnj.1 and miR-155-5p levels in retina harvested from I/R and sham group mice injected with scrambled siRNA or lncRNA uc007nnj.1 siRNA. E Representative images of double staining with Tuj1 antibody (green) and TUNEL (red) reagents on retinal sections. F Comparison of the ratio for the density of TUNEL positive RGCs to the total number of DAPI-stained nuclei in the ganglion cell layer in a different group. Scale bar: 100 μm. G Representative scotopic ERG traces under the intensity of 3.0 cd·s/m2 on day 1 and day 7 after I/R injury. H Statistical analysis of the b-wave amplitudes at 3.0 cd·s/m2 under dark-adapted conditions. I–J Representative Western blot images and densitometric measurements show the levels of cleaved caspase-3, caspase-3 and Tle4. K, L Representative immunolabeled images and quantitative analysis of Tle4 (green) on the RGC layer. Scale bar: 100 μm. Data are expressed as mean ± SD (n = 6). #p < 0.05, scramble/I/R group versus scramble/sham group; *p < 0.05, lncRNA uc007nnj.1 siRNA/I/R group versus scramble/I/R group