Fig. 3 

TOP2A overexpression promotes proliferation cell cycle, and inhibits apoptosis in trophoblasts. A Western Blot was used to test the efficiency of plasmid transfection. B mRNA levels of TOP2A were assessed in HTR-8/SVneo cells after plasmid transfection. C q-PCR was used to assess the mRNA level of Bax, an apoptosis-related gene, after TOP2A overexpression. D The effect of TOP2A overexpression on the protein levels of cleaved-caspase3 and caspase3 were analysed using western blot. E Apoptosis was assessed using flow cytometry analysis. F Flow cytometry analysis was used to compare differences in cell cycle in the two groups Each in vitro experiment was repeated three times, and data are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ****P < 0.0001. ns no statistical difference