Fig. 6

Downregulation of TOP2A affects the FOXO signaling pathway. A Western blotting showed changes in the levels of phosphorylated- FoxO1, phosphorylated-FoxO3a and phosphorylated-Rb after TOP2A downregulation. B Western blotting was used to assess changes in the level of phosphorylated-FoxO1 in the inhibitor-treated group. C q-PCR assay was used to verify changes in the mRNA expression of several downstream signaling molecules. D mRNA expression of cycle-associated genes was assessed after TOP2A knock down in JEG-3 cells and after treatment with chemical inhibitors that reduce FoxO1 activity. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001