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Fig. 5 | Molecular Medicine

Fig. 5

From: Bone marrow mesenchymal stem cell-derived exosomal lncRNA KLF3-AS1 stabilizes Sirt1 protein to improve cerebral ischemia/reperfusion injury via miR-206/USP22 axis

Fig. 5

The interaction of KLF3-AS1, miR-206and USP22. A Bioinformatics predicted the binding sites of several miRNAs with KLF3-AS1. Cells were transfected with pcDNA-KLF3-AS1 or control vector, then miRNAs levels were detected. B, C Luciferase activity examined using luciferase reporter assay. D Anti-Ago2 RIP assay in BV-2 cells was performed, then KLF3-AS1, miR-206 and USP22 mRNA associated with Ago2 were measured. E Cells were transfected with pcDNA-KLF3-AS1, RIP assay was then carried out. And the enrichment of KLF3-AS1 and USP22 was detected. F KLF3-AS1, miR-206 and USP22 mRNA levels were measured in cells transfected with pcDNA-KLF3-AS1. G, H Cells were transfected with KLF3-AS1 overexpression plasmid, vector or miR-206 mimic, then qRT-PCR (G) or western blot (H) was performed for measuring USP22. Values were expressed as mean ± SD of three separate determinations in the in vitro assays. *P < 0.05, **P < 0.01, ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658