Fig. 2

PLK1 overexpression ameliorates sepsis-induced intestinal injury. CAG-PLK1 mice or wild type mice were underwent CLP and then analysed. A Microphotographs of H&E-stained intestine sections from the indicated groups. The scale bar represents 100 μm. The Chiu’s score of each group shown right. B Serum concentrations of DAO in each group. C The levels of ZO-1 and Occludin were analysed by Western blotting. The graph shows the relative band densities. D Representative images of TUNEL staining after the indicated treatments. The graph shows the percentage of TUNEL-positive cells. The scale bar represents 50 μm. E The levels of apoptotic markers were measured by Western blots. The graph shows the relative band densities. F Morphological alterations in mitochondria were examined by TEM (scale bar = 1.0 μm). Yellow arrows indicate the relatively normal mitochondrial shape in the sham group. Red arrows denote deformed mitochondria with the loss of clearly defined cristae. Green arrows indicate rescued mitochondrial shape. G The relative fluorescence intensity of ROS in the intestine. H The ratio of JC-1 red/green fluorescence intensity which reflecting the MMP level was shown in graph. I Representative Western blots showing mitochondrial fusion (Mfn1, Mfn2, OPA1) and fission (Drp1 and Fis1) on the left, and the right graph show the relative band densities. The data in the graphs are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001 (n = 6)