Fig. 5

LINC00963 stabilized Zeb1 by modulating its ubiquitination. Expression levels of Zeb1, Snail, Slug, and Twist in LINC00963 overexpressed cells were examined by RT-qPCR (A) and western blot (B). C Cytoplasmic and nuclear fractions were prepared from A549 and NCI-H1650 cells. Zeb1 expression was examined by western blot. D Stability of Zeb1 protein was examined by CHX chase assay in LINC00963 overexpressed A549 cells. E Ubiquitinated Zeb1 was measured by Co-IP assay in LINC00963 overexpressed A549 cells. F Migration and invasion were assessed by Transwell assays in indicated A549 cells. Scale bar: 100 μm. G Western blot was performed to test Zeb1, E-cadherin, and N-cadherin protein levels. *P < 0.05, **P < 0.01 and ***P < 0.001