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Fig. 6 | Molecular Medicine

Fig. 6

From: LINC00963 promotes the malignancy and metastasis of lung adenocarcinoma by stabilizing Zeb1 and exosomes-induced M2 macrophage polarization

Fig. 6

LINC00963 triggered the degradation of Siah1 mRNA to induce up-regulation of Zeb1. A Expression levels of indicated E3 ubiquitin ligases were examined by RT-qPCR in LINC00963 overexpressed cells. B Siah1 protein level was examined by western blot in LINC00963 overexpressed cells. C Expression levels of Zeb1 and Siah1 were examined by western blot in indicated cells. D The potential interaction between LINC00963 and Siah1 was examined by RIP assay using anti-Siah1 and IgG (negative control) antibody. E The mRNA stability of Siah1 was examined by RT-qPCR after treating Actinomycin D for indicated time periods in LINC00963 overexpressed cells. n.s., not significant, *P < 0.05, **P < 0.01 and ***P < 0.001

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Molecular Medicine

ISSN: 1528-3658