Fig. 5

The function of ACK1 in lung cancer cells. A Expression of ACK1 in human lung bronchial epithelial (Beas-2B) and indicated lung cancer cell lines. B, C RT-qPCR B and western blot analyses C were used to verify ACK1 at mRNA and protein levels in A549 cells infected with lenti-shACK1 and NCI-H1299 cells with empty vectors and ACK1 overexpression lentiviral vectors. The black arrowhead indicated the ACK1 bands in the blot. The colony formation D and CCK8 E assays showed the effect of ACK1 inhibitor (AIM-100) on A549 cell viability and proliferation capacity, respectively. Cells were allowed to grow for ten days to form visible colonies. F, G The impact of AIM-100 on apoptosis of A549 cells at 24 h after treatments. H, I After an incubation of 18 h, the effects of knockdown and overexpression of ACK1 on cell migration were measured using a transwell assay in A549 H and NCI-1299 I NSCLC cells, respectively. *P < 0.05, **P < 0.01, ***P < 0.001