Fig. 8

Chloroquine (CQ, 20 μM) or Bafilamycine A1 (BafA1, 20 nM) sensitized lung cancer cells to ACK1 inhibition by AIM-100 (20 μM). A A549 cells were treated with CQ, AIM-100, or in combination for 48 h, and cellular viability was measured using CCK8 assay. B CCK8 assay was performed on A549 cells with the replacement of CQ with BafA1. C, D After A549 cells were treated as A and B for ten days, colony formation was evaluated for each group. E–H Impacts of indicated treatments on A549 cells’ migration abilities were assessed by wound-healing (E, F) and transwell migration assay (G, H), which were determined after exposure to noted drugs for 18 h. *P < 0.05, **P < 0.01, ***P < 0.001