Fig. 2
Construction of ER stress model in HTR-8/SVneo cells where METTL3 expression and m6A level were upregulated. A Cell viability determined by the CCK8 analysis. B–D Representative WB images and densitometry quantification of GRP78 and CHOP. The data were normalized to β-actin. E Flow diagram of constructing ER stress model. HTR-8/SVneo cells were treated for 6 h with 100 nmol/L TG and another 18 h with fresh medium and used for the following experiments. F–I Representative WB images and densitometry quantification of METTL3, FTO and METTL14. The data were normalized to β-actin. J The m6A levels determined by m6A colorimetric assay. n = 3. ns: no significance, *p < 0.05, **p < 0.01, ***p < 0.001