Fig. 3
Attenuated ER stress, decreased ROS production and apoptosis rate in TG-treated HTR-8/SVneo cells by knockdown of METTL3. A The knockdown efficiency of different sh-METTL3s determined by qRT-PCR. B The m6A level in different groups determined by m6A colorimetric assay. C–F Representative WB images and densitometry quantification of METTL3, GRP78 and CHOP. The data were normalized to β-actin. G, H ROS production detected by DCFH-DA. Photographs were obtained at 100 × (Scale bar 100 μm). I, J Apoptosis rate in different groups detected by flow cytometry. K Representative transwell photos of HTR-8/SVneo cells in different groups (100 × magnification, Scale bar 100 μm). L Qualified data shown in K. n = 3. *p < 0.05, **p < 0.01, ***p < 0.001