Fig. 4
The relationship between METTL3 and TMBIM6 in HTR-8/SVneo cells under ER stress. A The relative expression levels of TMBIM6 mRNA in different groups detected by qRT-PCR. B, C Representative WB images and densitometry quantification of TMBIM6 in different groups. The data were normalized to β-actin. D The m6A level of TMBIM6 mRNA in different groups measured by MeRIP-qPCR. E TMBIM6 expression determined by IHC. Photographs were obtained at 100 × (up, Scale bar 100 μm) and 200 × (down, Scale bar 50 μm) magnification. F Qualified data shown in E. G, H Representative WB images and densitometry quantification of TMBIM6 in the placentas from preeclamptic and healthy pregnant women. The data were normalized to β-actin. I The relative expression levels of the TMBIM6 mRNA in healthy and preeclamptic placentas determined by qRT-PCR. H: Healthy Pregnant Women; P: Preeclamptic Pregnant Women. n = 3. *p < 0.05, **p < 0.01