Fig. 5

Attenuated TG-induced ER stress, oxidative stress and apoptosis in HTR-8/SVneo cells by overexpressing TMBIM6. A–H Representative WB images and densitometry quantification of TMBIM6, GRP78, CHOP, NRF2, HO-1, Bax and Bcl-2 in different groups. The data were normalized to β-actin. I, J ROS production detected by DCFH-DA. Photographs were obtained at 100 × (Scale bar 100 μm). K, L Apoptosis rate in different groups detected by flow cytometry. M Representative transwell photos of HTR-8/SVneo cells in different groups (100 × magnification, Scale bar 100 μm). N Qualified data shown in M. Oe-Con: Overexpression of control plasmid; Oe-TMBIM6: Overexpression of TMBIM6 plasmid. n = 3. *p < 0.05, **p < 0.01