Fig. 6

Overexpression of TMBIM6 partially neutralized the effects of METTL3 on TG-induced ER stress, oxidative stress and apoptosis in HTR-8/SVneo cells. A–I Representative WB images and densitometry quantification of METTL3, TMBIM6, GRP78, CHOP, NRF2, HO-1, Bax and Bcl-2 in different groups. The data were normalized to β-actin. J, K ROS production detected by DCFH-DA. Photographs were obtained at 100 × (Scale bar 100 μm). L, M Apoptosis rate in different groups detected by flow cytometry. N Representative transwell photos of HTR-8/SVneo cells in different groups (100 × magnification, Scale bar 100 μm). O Qualified data shown in L. Oe-Con: Overexpression of control plasmid; Oe-METTL3: Overexpression of METTL3 plasmid; Oe-TMBIM6: Overexpression of TMBIM6 plasmid. n = 3. *p < 0.05, **p < 0.01, ***p < 0.001