Fig. 8
The therapeutic effect of inhibiting METTL3 in preeclamptic rat. A The flow diagram of our animal experiments. B The sperm harvested from the vagina of pregnant rats and observed under a light microscope on GD1. C, E The SBP measured on GD15 and GD20. D, F The urinary protein measured on the GD15 and GD20. G, H The photos of fetuses and the fetal weight on GD20 in different groups. I, J The photos of placentas and the placental weight on GD20 in different groups. K METTL3 expression in the placentas from preeclamptic and healthy pregnant rats determined by IHC. Photographs were obtained at 200 × (Scale bar 50 μm) magnification. L Qualified data shown in K. M The successful transfection into placentas with LV containing METTL3 shRNA indicated by the green fluorescence. Photographs were obtained at 100 × (Scale bar 100 μm) magnification. N–P The knockdown efficiency of METTL3 shRNA measured by qRT-PCR and WB. Q The pathological changes of kidneys from different groups indicated by HE staining. Photographs were obtained at 200 × (Scale bar 50 μm) magnification. S The different m6A levels in the placentas from different groups determined by m6A colorimetric assay. R, T–Z Representative WB images and densitometry quantification of TMBIM6, GRP78, CHOP, NRF2, HO-1, Bcl-2 and Bax in different groups. The data were normalized to β-actin. LV-sh-Con: lentivirus containing control shRNA; LV-sh-METTL3: lentivirus containing METTL3 shRNA. n = 3. ns: no significance, *p < 0.05, **p < 0.01, ***p < 0.001