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Fig. 2 | Molecular Medicine

Fig. 2

From: AAV-glycine receptor α3 alleviates CFA-induced inflammatory pain by downregulating ERK phosphorylation and proinflammatory cytokine expression in SD rats

Fig. 2

Effects of prostaglandin EP2-receptor antagonist (PF-04418948), glycine-receptor antagonist (strychnine), and PKC inhibitor (G06983) on PGE2-induced ERK phosphorylation. A Time schedule in which F11 cells were seeded into a 6-well plate for 24 h; PF-04418948 (10 μM) or strychnine (10 μM) was applied and incubated for 24 h before PGE2 treatment. G06983 (3 μM) was applied 30 min before PGE2 treatment. One hour after PGE2 (100 μM) application, cells were harvested for protein extraction. B Western blotting of ERK phosphorylation and C quantification of ERK phosphorylation in F11 cells. F11 cells without inhibitors or PGE2 treatment were used as a control. The data are presented on the basis of from three to five independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001, one-way ANOVA

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Molecular Medicine

ISSN: 1528-3658