Fig. 1

PS-OMe miR130 decreases eCIRP mediated inflammation in vitro. A Biotinylated PS-OMe miR130 was individually immobilized on a sensor chip SA, and eCIRP of varying concentrations was injected as the analyte (n = 3 independent SPR experiments). B Computational 3D-modeling illustrating docking of PS-OMe miR130 and eCIRP. RAW264.7 cells (1 × 10⁶ cells/ml) were treated with PBS (Ctrl), eCIRP (1 µg/ml) with 0 or 10 nM of PS-OMe mIR130 (miR). After 24 h, supernatant was collected and C TNF-α levels were measured. Mouse peritoneal macrophages (1 × 10⁶ cells/ml) were treated with PBS, eCIRP (1 µg/ml), or eCIRP (1 µg/ml) with 10 nM of PS-OMe miR130 (miR). After 24 h, supernatant was collected and D TNF-α and E IL-6 were measured (n = 5 replicates/group). Data expressed as means ± SD and compared by one-way ANOVA and SNK method (*P ≤ 0.05 versus Ctrl; ^#P ≤ 0.05 vs. eCIRP)