Fig. 2
From: An engineered miRNA PS-OMe miR130 inhibits acute lung injury by targeting eCIRP in sepsis
Stability of PS-OMe miR130 in vitro and in vivo. A 8 µM of PS-OMe miR130 was incubated in DMEM containing 10% non-heat-inactivated FBS for different time points. Samples were run on a denaturing gel and stained with ethidium bromide and viewed via UV light (n = 3 independent stability experiments). B In vivo half-life was determined by measuring the fluorescence in serum at different timepoints after injecting PS-OMe miR130 labeled with 3′ cy-3. Fluorescence was measured at each time point. C The natural logarithm (Ln) of the fluorescence was graphed over the elimination phase time-period. The slope was determined and using the slope, the elimination half-life was calculated (n = 4)