Fig. 6
From: An engineered miRNA PS-OMe miR130 inhibits acute lung injury by targeting eCIRP in sepsis
PS-OMe miR130 ameliorates lung injury. Lung tissue was collected for sham, vehicle (veh), and PS-OMe miR130 (miR) treated mice at 20 h after CLP. The right lower lobe was stored in 10% formalin. A Representative images of hematoxylin and eosin sections are shown at 200x. Scale bar 100 μm. B Lung injury was graded using a system created by the American Thoracic Society (n = 5–6/group). Sections were subjected to TUNEL assay to detect DNA fragmentation. TUNEL staining (green fluorescent) and nuclear counterstaining (blue fluorescent) of lung sections are shown. C Representative images of TUNEL staining from sham, vehicle (veh), and PS-OMe miR130 (miR) shown at ×200 magnification. D TUNEL positive cells were counted using ImageJ software (n = 5–6/group). Data expressed as means ± SEM and compared by one-way ANOVA and SNK method (*P ≤ 0.05 versus sham; #P ≤ 0.05 vs. veh)