Fig. 1

HMA effectively inhibits the proliferation capacity of osteosarcoma cells: The MNNG/HOS and U-2 OS cells were treated with different concentrations of HMA (0, 0.1, 1, 5, 10, 25, 50, 100, and 200 μmol/L) for A CCK-8 assay to evaluate the viability of the OS cells. Treatment with 0, 50, 100, and 200 μmol/L HMA of the OS cells was conducted for B colony formation assay, C, D Annexin V-FITC apoptosis detection assay, E Western blotting, and F, G transwell assay to detect the various biological behaviors of the OS cells in vitro. H The MNNG/HOS cells were injected subcutaneously into nude mice for xenograft assay. I Tumor growth curve and J excised tumor mass, as indicated. (K) IHC scores for Ki-67 staining, as indicated