Fig. 5
HMA impairs OS proliferation through the MYC-CHK1-RAD51 axis: A Venn diagram showing the intersection genes between the downstream genes of MYC and downregulated genes in RNA-sequencing. B The IC50 of AZD7762 was detected in the MNNG/HOS cells with or without HMA treatment. After treating with AZD7762 and HMA, the cells were analyzed using C, D Western blotting and E H2A.X immunofluorescence. After treating with si-MYC and HMA, the cells were analyzed using F PCR and G, H Western blotting. I CHIP–qRT-PCR assay was used to detect the enrichment efficiency of MYC on the CHK1 promoter