Fig. 3

The expression of RBM15 was increased in F1 generation liver tissue. a The methylated RNA (m6A) level was determined in liver tissue of GD18.5 fetus of the GDM group and Control (n = 8) (**P < 0.01 by Student’s t-test); b PCR array of m6A methyltransferases, demethylases and effector proteins was measured in liver tissue of GDM group and Control (n = 8). Expression of log10 fold change relative to control mice. mRNA expression levels of METTL3 (c), RBM15 (d), IGF2BP1 (e) and IGF2BP2 (f) in GD18.5 fetus liver were examined using RT-qPCR (n = 12). Protein levels of both GD18.5 fetus (g) and F1 generation at the age of 12 weeks (h) were determined by western blot. i, j Image of IHC staining of RBM15 in GD18.5 fetus and 12-week offspring liver tissue respectively. Results are shown as mean ± SEM, *P < 0.05, ** P < 0.01, ***P < 0.001