Fig. 2

miR-16-5p overexpression induces foot wound healing in DM rats. A RT-qPCR detection of the miR-16-5p expression in the foot wound tissues of DM rats treated with agomir NC or miR-16-5p agomir. *p < 0.05 vs. the agomir NC-treated rats. B Skin wound diameter changes in DM rats treated with agomir NC or miR-16-5p agomir. * p < 0.05 vs. agomir NC-treated rats. C Morphological and structural changes of foot wound tissues observed by HE staining. D Masson staining of collagen deposition in the foot wound tissues of DM rats treated with agomir NC or miR-16-5p agomir. E The number of newly formed and mature vessels in foot wounds of DM rats treated with agomir NC or miR-16-5p agomir assessed by immunohistochemical and immunofluorescent staining. *p < 0.05 vs. agomir NC-treated rats. n = 8. Measurement data were presented as mean ± standard deviation. Data among multiple groups were compared by one-way ANOVA, followed by Tukey’s post hoc test for multiple comparisons. Repeated measures ANOVA with Tukey’s post hoc test was applied for the comparison of data at different time points