Fig. 5

SP5 is a target gene of miR-16-5p. A Binding sites between miR-16-5p and SP5 predicted by the starBase database. B RT-qPCR detection of SP5 expression in rat foot wound tissues of non-DM and DM rats. *p < 0.05 vs. normal rats; #p < 0.05 vs. non-DM rats. C The binding of miR-16-5p to SP5 in HEK293T cells validated by dual-luciferase reporter assay. #p < 0.05 vs. mimic NC-transfected HEK293T cells. D SP5 expression in fibroblasts transfected with miR-16-5p mimic or miR-16-5p inhibitor detected by RT-qPCR. *p < 0.05 vs. fibroblasts transfected with mimic NC; # p < 0.05 vs. fibroblasts transfected with inhibitor NC. E Western blots of SP5 protein in fibroblasts transfected with miR-16-5p mimic or miR-16-5p inhibitor. *p < 0.05 vs. fibroblasts transfected with mimic NC. #p < 0.05 vs. fibroblasts transfected with inhibitor NC. Cell experiments were repeated three times. n = 8. Measurement data were presented as mean ± standard deviation. Data among multiple groups were compared by one-way ANOVA, followed by Tukey’s post hoc test for multiple comparisons