Fig. 7

IRF1-exosome triggers fibroblast proliferation and migration, and endothelial cell angiogenesis via the miR-16-5p/SP5 axis. Fibroblasts or endothelial cells were treated with exosomes, IRF1-exosome or IRF1-exosome + oe-SP5. A and B, IRF1, miR-16-5p and SP5 expression in Rat2 fibroblasts (A) and YPEN-1 endothelial cells (B) measured by RT-qPCR. C Rat2 fibroblast proliferation determined by EdU. D Rat2 fibroblast migration determined by scratch test. E YPEN-1 endothelial cell angiogenesis as detected by vessel-like tube formation assay. *p < 0.05 vs. exosome-treated fibroblasts or endothelial cells. # p < 0.05 vs. IRF1-exosome + oe-NC-treated fibroblasts or endothelial cells. Cell experiments were repeated three times. Measurement data were presented as mean ± standard deviation. Data among multiple groups were compared by one-way ANOVA, followed by Tukey’s post hoc test for multiple comparisons