Fig. 3

Knockout of PINK1 prevented DC function following LPS treatment. Immature DCs from WT mice and pink1 Knockout mice were cultured by PBS or LPS (1000 ng/mL) for 4 h. a, b Representative western blots of PINK1 and COXIV in each group and statistical analysis of relative PINK1 expression (n = 3). c, d Representative flow cytometric analysis of MHC-II, CD80, and CD86 expression and statistical analysis of relative MHC-II, CD80, and CD86 expression on DCs in each group (n = 4). e TNF-α mRNA expression was analyzed by PCR and statistical analysis of relative mRNA expression of TNF-α on DCs in each group (n = 3). f IL-12 mRNA expression was analyzed by PCR and statistical analysis of relative mRNA expression of IL-12 on DCs in each group (n = 3). g CD4⁺ T cells were activated with ConA (5 μg/mL) for 18 h, and then were co-cultured with DCs at a ratio of 1:100. CCK-8 was used to test T cell proliferation (n = 5). Results of experiments were shown as the mean ± SD. Statistical significance was assessed using Student’s t test (b) and two-way ANOVA analysis with Sidak’s multiple comparisons test (d–g). P values are reported as follows: ^# < 0.05 and **, ^## < 0.01