Fig. 4

Mitohpgay induction in DCs at different time following LPS treatment. a, b Representative western blots of Parkin and COXIV in each group and statistical analysis of relative expression (n = 5). c, d Representative western blots of LC3, Tomm20 and β-actin in each group and statistical analysis of relative LC3II and Tomm20 expression (n = 5). e, f Representative fluorescence images of the co-localization of lysosome and mitophagy and statistical analysis of percentage of mitophagy-positive cells on DCs in sham and CLP group, including lysosome (green), mitophagy (yellow) and nucleus (blue) (n = 3). g Representative electron micrographs of mitophagosomes on DCs in Control and LPS group. Results of experiments were shown as the mean ± SD. Statistical significance was assessed using Student’s t test (f) and one-way ANOVA analysis with Dunnett’s multiple comparisons test (b, d). P values are reported as follows: ** < 0.01