Fig. 7

Knockout of PINK1 prevented DC function and increased apoptosis of DCs after CLP. Splenic DCs were collected from WT mice and PINK1 knockout mice at 24 h after CLP. a, b Representative flow cytometric analysis of MHC-II, CD80, and CD86 expression and statistical analysis of relative MHC-II, CD80, and CD86 expression on DCs in each group (n = 4). c TNF-α mRNA expression was analyzed by PCR and statistical analysis of relative mRNA expression of TNF-α on DCs in each group (n = 3). d IL-12 mRNA expression was analyzed by PCR and statistical analysis of relative mRNA expression of IL-12 on DCs in each group (n = 3). e CCK-8 was used to test T cell proliferation (n = 5). f, g Representative images of BrdU staining (red) and DAPI (blue) on DCs in each group and statistical analysis of positive cells (n = 3). h, i Apoptosis of DCs was tested by PE-Annexin V and 7-AAD. Representative flow cytometric analysis of apoptosis and statistical analysis of DCs apoptosis in each group (n = 3). j, k Representative western blots of cleaved caspase-3 and β-actin in each group and statistical analysis of relative cleaved caspase-3 expression (n = 3). l Survival rate of the mice from each group over 7 days. Results of experiments were shown as the mean ± SD. Statistical significance was assessed using two-way ANOVA analysis with Sidak’s multiple comparisons test. P values are reported as follows: *, ^# < 0.05 and **, ^## < 0.01